ICSI or Intracytoplasmic Sperm Injection
ICSI or intracytpolasmic sperm Injection is the most popular form of test tube baby treatment carried out by most IVF centres in India.
A majority of the IVF centres in Mumbai have an ICSI for all patients policy.
This procedure differs from IVF in the method of fertilization of the egg and the sperm.
This procedure involves stimulating the ovaries with the help of hormonal injections for approximately 11 to 12 days and doing a daily or alternate day sonography for the patient.
We simultaneously try and stimulate as many follicles as is possible for the woman, and based on her age and AMH this can range from1 or 2 to 25 follicles as well.
Around the 14th day of the period, the woman is admitted in the hospital for about 6 hours for ovum pick up or egg retrieval procedure. The procedure is safe and does not involve any stitches being administered.
The best ivf centres in Mumbai usually do not keep the patient overnight and the patient is discharged around 4 hours after the egg retrieval procedure. Hence ivf treatment in Mumbai can be labeled as a day care procedure.
Our ivf centre in Mumbai, believes in a minimum discomfort policy and our patients undergoing ivf treatment can continue to work right until the day of embryo transfer.
Once the eggs have been retrieved from the ovaries, the husbands semen sample is collected in a container and is washed and processed.
After sperm washing all the sperms are magnified under an inverted microscope at 400X magnification and the best sperms are selected and segregated.
Unlike IVF where one egg is placed in the middle of 50000 sperms, in ICSI a single best sperm is selected for each egg. Then with the help of an instrument called the micromanipulator, the selected sperm is injected directly into the egg.
The resultant embryos that develop can be cultured to day 3 stage or to blastocyst stage (day 5) before being transferred back into the uterus.
A successful implantation can be confirmed with the help of a blood test 12 days after the embryo transfer procedure.